trna synthetase meaning in English
trna合成酶
Examples
- Polymyositis ( pm ) and dermatoyositis ( dm ) , devastating inflammatory muscle diseases , are associated with the myositis - specific anti - jo - 1 autoantibody , found in 25 - 40 % of such patients . anti - jo - 1 antibodies react with histidyl - trna synthetase
抗组氨酰? trna合成酶抗体，临床上又称抗jo - 1抗体，是诊断这类疾病的标志性抗体。 - Hisityl - trna synthetase catalyzes the aminoacylation of trnahis in the initial step of protein biosynthesis . the involumen of histidyl - trna synthetase in autoimmune diseases is another feature of the enzyme . in studies , it is reported that purified jo - 1 antigen can increase the detection rate of anti - jo - 1 antibody . but it is difficult to obtain a single component by biochemical extraction . genetic engineering can help us to desolve this problem . after looking up mrna sequence encoding histidyl - trna synthetase in genebank , we used rt - pcr technology to gain its full length dna sequence . the vestor ptybllwas used in the construction of expressing vestor . we transformed the jo - 1 gene into er2566 and used this system to express fusion jo - 1 antigen
本实验从人胎盘中提取总rna ，利用rt - pcr技术获得了编码jo - 1的基因整长序列，选用impact - cn系统中的ptyb11载体，构建了jo - 1基因的克隆与表达载体，并转化大肠杆菌er2566 ，经过抗性筛选、分子量大小比较、双酶切鉴定、和pcr鉴定等多种方法验证，筛选出了5个阳性克隆。 - A page gel , stained by the petiodic acid - schiff ' s method to reveal glycoproteins , further displayed that the bindng - protein was a glycoprotein belonging to lectin , which contained 17 . 4 % ( w / w ) neutral carbohydrate content of the glycoprotein detected by the phenol / h2so4 method . peptide mapping was comparable to the reported protein in protein bank . the database homology search ( ncbi blast ) indicated that the binding - protein shared 70 - 80 % homogeneity to l - aspartate oxidase , aspartyl / glutamyl - trna ( asn / gln ) amidotransferase subunit b , glutamyl - trna reductase , histidyl - trna synthetase
连续梯度聚丙烯耽胺凝胶电泳、 sds一聚丙烯酞胺凝胶电泳和等电聚焦的结果表明该蛋白分子量为1 “ . skda ，由二个相同的亚基组成，亚基分子量为” . ikda ，等电点为8 . 25 .糖蛋白染色结合考马斯亮蓝染色的结果证实此结合蛋白是个糖蛋白，其中糖含量为17 . 44 % ，蛋白含量为82 . 56 % .凝集反应确定该糖蛋白也是一个凝集素